1. CLEAN THE TAP
Remove from the tap any attachment that may cause splashing and using a clean cloth or cotton immersed in alcohol, wipe the outlet in order to remove.
2. OPEN THE TAP
Turn on the tap at maximum flow rate and let the water flow for 1-2 minutes.
3. STERILIZE THE TAP
Sterilize the tap for a minutes with the flame from an ignited cotton ball soaked in alcohol: alternatively, a gas burned or cigarette lighter maybe used.
4. OPEN THE TAP PRIOR
Carefully turn on the tap and allow the water to flow for 12 minutes at a medium flow rate.
5. OPEN A STERILIZED BOTTLE
Remove the white paper cover and unscrew the bottle cap.
6. FILL THE BOTTLE
While holding the cap and protective cover ace downwards (so as to prevent entry of dust that might carry micro-organisms) immediately hold the bottle under the water faucet and fill.
A small air space should be left to facilitate shaking at the time of inoculation prior to analysis.
7. CAP THE BOTTLE
Screw the cap on the bottle and fix the white paper protective covering.